# SPRM Outputs The following are descriptions of SPRM outputs related to their reg expression naming (xxx). The outputs can be grouped into x types ### OME-TIFFs showing pixel level results (remapping of channels) [3 per input image] - xxx-channel_pca.ome.tiff - xxx-superpixel.ome.tiff ### CSV showing interpolated cell outlines & polygons [2 per input image] - xxx-cell_shape.csv - xxx-cell_polygons_spatial.csv ### CSVs containing features for each cell [4 per image] - xxx_texture.csv - xxx_cell_channel_meanALL.csv - xxx-cell_adj_list.csv - xxx-cell_centers.csv ### CSVs containing features for each segmentation [12 per image] - xxx-[segmentation channel]_channel_covar.csv - xxx-[segmentation channel_channel_mean.csv - xxx-[segmentation channel]l_channel_total.csv ### Master CSV containing clustering results for each cell (row) for each method (column) [1 per input image] - xxx-cell_cluster.csv - ### CSVs containing mean values of “markers” for each cluster for each clustering method [5 per input image] - xxx-clustercells_cellcovariance_legend.csv - xxx-clustercells_cellmeanALL_legend.csv - xxx-clustercells_cellmean_legend.csv - xxx-clustercells_cellshape_legend.csv - xxx-clustercells_celltotal_legend.csv ### PNGs showing coloring by cluster [10 per input image] - xxx-ClusterByCovarPerCell.png - xxx-ClusterByMeansAllMasks.png - xxx-ClusterByMeansPerCell.png - xxx-ClusterByShape.png - xxx-ClusterByTotalPerCell.png - xxx-Superpixels.png - xxx-Top3ChannelPCA.png - xxx_nmf_top3.png - xxx-ClusterBytSNEAllFeatures.png - xxx-ClusterByUMAP.png ### CSV containing the signal to noise ratios of the image per channel [1 per input image] - xxx-SNR.csv ### CSV containing PCA and Silhouette analysis of the image [2 per input image] - xxx-channelPCA_summary.csv - xxxclusteringsilhouetteScores.csv ### CSV containing the summary file [3 per run] - summary_otsu.csv - summary_zscore.csv - SPRM_Image_Quality_Measures.json